The Isolation of Plant Cuticle with Pectic Enzymes.
نویسنده
چکیده
corn root tip. Iowa State College Jour. Sci. 28: 177-188. 1953. 5. HOGEBOOM, G. H. and SCHNEIDER, W. C. Cytochemical studies. IV. Physical state of certain respiratory enzymes of mitochondria. Jour. Biol. Chem. 194: 513-519. 1952. 6. JAMES, W. 0. The terminal oxidases in the respiration of the embryos and young roots of barley. Proc. Roy. Soc. (London) B 141: 289-299. 1953. 7. KOCH, F. C. and MCMEEKIN, T. L. A new direct nesslerization micro-Kjeldahl method and a modification of -the Nessler-Folin reagent for ammonia. Jour. Amer. Chem. Soc. 46: 2066-2069. 1924. 8. KUGLER, 0. E. and BENNETT, E. H. Histochemical localization of acid phosphatase in germinating maize kernels. Stain Technol. 22: 9-15. 1947. 9. MAXWELL, R. E. Cytochrome oxidase in corn embryos. Plant Physiol. 25: 521-524. 1950. 10. MCCLENDON, J. H. The intracellular localization of enzymes in tobacco leaves. II. Cytochrome oxidase, catalase, and polyphenol oxidase. Amer. Jour. Bot. 40: 260-266. 1953. 11. MORRIS, H. J., WEAST, C. A., and LINEWEAVER, H. Seasonal variation in the enzyme content of eleven varieties of carrots. Bot. Gaz. 107: 362372. 1946. 12. PONTING, J. D. and JOSLYN, M. A. Ascorbic acid oxidation and browning in apple tissue extracts. Arch. Biochem. 19: 47-63. 1948. 13. ROCA, J. and ONDARZA, R. The enzymic activity during the process of germination. V. Catalase in varieties of maize. Chem. Abstracts 48: 4639h. 1954; Anales inst. biol. 24: 15-21. 1953. 14. SOMMER, A. J. The determination of acid and alkaline phosphatases using p-nitrophenyl phosphate as substrate. Medical Bulletin of St. Louis University 4: 165-169. 1952. 15. STAFFORD, H. A. The distribution and development of enzymes in pea seedlings. Bull. Torrey Bot. Club 79: 351-358. 1952. 16. SUMNER, J. B. and SOMERS, G. F. Chemistry and Methods of Enzymes, 3rd ed. Pp. 1462. Academic Press, New York. 1953. 17. TAUBER, H. Oxidation of pyrogallol to purpurogallin by crystalline catalase. Jour. Biol. Chem. 205: 395-400. 1953. 18. VAN FLEET, D. S. Histochemical localization of enzymes in vascular plants. Bot. Rev. 18: 354398. 1952. 19. WEIER, T. E. and STOCKING, C. R. The chloroplast: structure, inheritance, and enzymology. II. Bot. Rev. 18: 14-75. 1952.
منابع مشابه
PECTIC ENZYME PATTERNS OF FUSARIUM OXYSPORUM VIRULENT ISOLATES FROM CHICKPEA IN IRAN
The pectic enzymes produced in vitro by 8 isolates (5 Highly virulent and 3 Weakly virulent) of Fusarium oxysporum , were detected by spectrophotometry, and characterized by polyacrylamide gel electrophoresis with substrate-containing gels (zymogram). Analysis of the polygalacturonase (PG) isozyme banding patterns (zymogram) identified two distinct phenotypes among the isolates from chickpea (C...
متن کاملIsolation of Plant Cuticles With Zinc Chloride-hydrochloric Acid Solution.
rhe cuiticle of plants is a heterogenous memnbranie in which wax, pectin and cellulose in varying l)ro)ortions are contained in a cutin franmework. In the isolation of cuticular membranes from leaves or fruits the wax is nornially removed by extraction xvith ani organic solvent before detachment of the memnbranes from the underlying epidermal cells. Detachnmenit is achieve(d by the use of eithe...
متن کاملTransgenic Arabidopsis plants expressing a fungal cutinase show alterations in the structure and properties of the cuticle and postgenital organ fusions.
A major structural component of the cuticle of plants is cutin. Analysis of the function of cutin in vivo has been limited because no mutants with specific defects in cutin have been characterized. Therefore, transgenic Arabidopsis plants were generated that express and secrete a cutinase from Fusarium solani f sp pisi. Arabidopsis plants expressing the cutinase in the extracellular space showe...
متن کاملThe Wheat Grain Contains Pectic Domains Exhibiting Specific Spatial and Development-Associated Distribution
Cell walls are complex structures surrounding plant cells with a composition that varies among species and even within a species between organs, cell types and development stages. For years, cell walls in wheat grains were described as simple walls consisting mostly of arabinoxylans and mixed-linked beta glucans. Proteomic and transcriptomic studies identified enzyme families involved in the sy...
متن کاملPectic Acid Effects on Prolactin Secretion in GH3/B6 Rat Pituitary Cell Line
Background: Pectic acid extracted from plants increases the secretion of prolactin (PRL) when injected intravenously into ewes or fed to rats. Fragments of ewe hypophysis and lactating rabbit mammary gland incubated in vitro in the presence of pectic acid secreted more PRL and caseins compared to the controls. However, it is not known whether pectic acid directly stimulates PRL secretion in pi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Plant physiology
دوره 30 1 شماره
صفحات -
تاریخ انتشار 1955